Lactic dehydrogenase activity in human epidermis: semiquantitative histochemical study.

SASAI, Y. Lactic Dehydrogenase Activity in Human Epidermis: Semiquantita tive Histochemical Study. Tohoku J. exp. Med ., 1972, 108 (4), 327-336 The graphical method of Lineweaver-Burk to the histoehemical system was applied to obtain quantitative information on the lactic dehydrogenase activity in human epidermis. In vitro experiment showed that the incubation time necessary for yielding a certain amount of formazan is related to the amount of enzyme present. In the case of section experiment, the time required for the first appearance of formazan deposition in the tissue at various substrate concentra tions was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the lactic dehydrogenase activities at various sites of the tissue. lactic dehydrogenase; human epidermis; semiquantitative histochemistry